In vitro Transcription by RNA Polymerase Associated with Rice Gall Dwarf Virus
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چکیده
An RNA-dependent polymerase associated with rice gall dwarf virus (RGDV), a new Phytoreovirus, was characterized. Opt imum polymerase activity was found between pH 8.0 and 8.5 at 25 °C. Mg 2+ was essential for the activity, the opt imum concentration being 2 mM. Mn 2 + could replace Mg 2 +. The R N A products synthesized in vitro hybridized with R G D V RNA, but not with R N A of rice dwarf virus which is in the same subgroup of plant reoviruses. Electrophoretic analysis of the products showed at least nine bands, the sizes of which are discussed.
منابع مشابه
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